BMEM Cardiomyocyte Selection
BMEM Cardiomyocyte Selection is a ready-to-use, serum-free formulation designed to selectively enrich cardiomyocytes from differentiated human iPSCs. Optimized for high purity and reproducibility, this medium creates a metabolic environment that supports the survival of cardiomyocytes while eliminating undifferentiated and non-cardiac cell types.
With streamlined protocols and consistent performance, BMEM Cardiomyocyte Selection Media enables researchers to obtain highly enriched cardiomyocyte populations for downstream applications, including disease modeling, drug discovery, and regenerative medicine research.
Key Features:
- Robust cardiomyocyte enrichment from hiPSC-derived cultures
- Serum-free, xeno-free formulation for consistency and scalability
- Easy-to-use protocol requiring no specialized equipment
- High reproducibility across multiple iPSC lines
- Compatible with downstream functional assays and long-term culture
View PDF
Instructions for Use
BMEM Cardiomyocyte Selection is ready-to-use. Bottles can be used straight from the refrigerator (4 °C) and should not be warmed to RT or 37 °C prior to use.
Directions for Use
BMEM Cardiomyocyte Selection is designed for the purification of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) following differentiation. Selection is typically
initiated between Day 10 –14 of differentiation, when cardiomyocytes are contracting and non-cardiomyocyte populations remain present. After selection, enriched cardiomyocytes can be maintained long-term in BMEM Cardiomyocyte Maintenance.
- On d10 (d14 the latest) of differentiation, verify your population of hiPSC-CMs with visible spontaneous contractions.
- Aspirate BMEM Cardiomyocyte Maintenance media and replace with BMEM Cardiomyocyte Selection.
- After 48 hours aspirate all media and replace with BMEM Cardiomyocyte Selection.
- After 48 hours aspirate all media and replace with BMEM Cardiomyocyte Maintenance.
Purified populations of hiPSC-CMs should be seen by d14. After selection, BMEM Cardiomyocyte Selection can be aspirated and replaced with BMEM Cardiomyocyte Maintenance. Cardiomyocytes can be maintained in BMEM Cardiomyocyte Maintenance for >45 days.
We recommend replating hiPSC-CMs to new wells for assays between d16 and d20.
Product Use
For Research Use Only.
Not for diagnostic procedures.
1. Cells do not seem to purify.
Cause: Cardiomyocyte purity was too poor. BMEM Cardiomyocyte Selection works best on cardiomyocytes thatare >50% pure.
- Solution: Complete new differentiation.
2. Cells cease to beat during selection.
Cause: Cardiomyocyte purity was too poor, and only a small population was comprised of contractile cardiomyocytes.
- Solution: Aspirate the selection media and add BMEM Cardiomyocyte Maintenance. If cells don’t start spontaneously beating after 24 h, complete a new differentiation.
To request the quality certificate use this link.
