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BMEM Protein-Free Cardiomyocyte Differentiation Kit

BMEM Protein-Free Cardiomyocyte Differentiation Kit

Optimized cardiomyocyte differentiation for human iPSC

Catalog #1003

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$350.00 USD
$350.00 USD
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BMEM Protein-Free Cardiomyocyte Differentiation Kit is a ready-to-use, highly optimized, chemically defined, animal origin-free (AOF) kit for the differentiation of human induced pluripotent stem cells (hiPSCs) to contracting cardiomyocytes (hiPSC-CM) in adherent culture.

This kit is a novel formulation optimized without any proteins. Differentiation using this kit typically produces contracting cardiomyocytes in 8-9 days and results in ~85-90% TNNT2+ cells and yields of >1x105 cells per cm2. Cardiomyocytes can be maintained in BMEM Cardiomyocyte Maintenance Medium for >45 days. 

BMEM Protein-Free Cardiomyocyte Differentiation Kit is compatible with hiPSC growth media BMEM Human Primed Pluripotent (1001) and BMEM Max Pluripotent (1004). 

BMEM Protein-Free Cardiomyocyte Differentiation Kit is compatible with a variety of culture matrices including: Corning® Matrigel® GFR, Gibco™ Geltrex™ GFR, R&D Systems™ Cultrex™ UltiMatrix GFR, SigmaAldrich® ECMatrix™-511 E8 Laminin, Gibco™ Recombinant Vitronectin, Corning® Synthemax II-SC, Acro Biosystems™ Human Laminin 521 Protein.

Each lot of BMEM Protein-Free Cardiomyocyte Differentiation Kit is performance tested on hiPSC, and sterility tested. 

Larger unit sizes are available on request.

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Product Information

The following components are sold as part of the BMEM Protein-Free Cardiomyocyte Differentiation Kit and are not available separately.
Component Name Size Storage
BMEM Cardiomyocyte d0 100 mL Store at 4°C
BMEM Cardiomyocyte d1 100 mL Store at 4°C
BMEM Cardiomyocyte d2 100 mL Store at 4°C
BMEM Cardiomyocyte Maintenance 2 x 500 mL Store at 4°C

Instructions/Directions for Use

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Instructions for Use

BMEM Protein-Free Cardiomyocyte Differentiation Kit is ready to use. Bottles can be used straight from the refrigerator (4 °C) and should not be warmed to RT or 37 °C prior to use. 

Directions for Use

hiPSC should be growing on a consistent schedule achieving 70-80% confluence before differentiation. Fast, consistent growth, without overgrowth (>90% confluence) is essential for reproducible differentiation. We recommend that hiPSC lines are ≥p20 for reproducible differentiation. For consistency, media changes should be completed at the same time of day.

  1. Grow hiPSCs to 70-80% confluence, typically this takes 4 days for cells cultured in BMEM or BMEM Max.
  2. Aspirate pluripotent media and replace with BMEM Cardiomyocyte d0.
  3. After 24 hours aspirate media and replace with BMEM Cardiomyocyte d1.
  4. After 24 hours aspirate media and replace with BMEM Cardiomyocyte d2.
  5. After 48 hours aspirate media and replace with BMEM Cardiomyocyte Maintenance Media.
  6. Every 48 hours aspirate media and replace with BMEM Cardiomyocyte Maintenance Media.

Contracting cardiomyocytes should be seen after 8-9 days.

We recommend replating hiPSC-CMs to new wells for assays between d16 and d20.

NOTE: Each cell line might require a different confluence level at the start of the protocol for a successful differentiation. Hence, we recommend testing different split ratios on the passage immediately before to assess which one is conducive to obtaining cardiomyocytes.

Product Use

For Research Use Only. 

Not for diagnostic procedures.